Figure 1
From: Identification of the angiogenic gene signature induced by EGF and hypoxia in colorectal cancer
Scatter plot of PCR angiogenesis array analysis of Caco-2 cells exposed to hypoxia and the hypoxia mimetic DMOG. Caco-2 cells were exposed to either (a) hypoxia (1% O2) or (b) DMOG (1 mM) for 24 hours. Scatter plot graphs are 2-ΔCt values for genes expressed by Caco-2 and normalised against HKG ActB (β-actin), 18S rRNA, HPRT1 (hypoxanthine phosphoribosyltransferase 1) and RPL13A (60S ribosomal protein L13a). Solid lines show no change, dashed lines show ≥2-fold increase and decrease versus untreated cells. Genes whose expression in both treated and untreated samples was below detection limits of the array are not included. Only genes whose expression changed ≥2-fold are annotated. Data are from a representative array performed in duplicate using cDNA pooled from 3 different replicate experiments.