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Figure 7 | BMC Cancer

Figure 7

From: CD26 Expression on T-Anaplastic Large Cell Lymphoma (ALCL) Line Karpas 299 is associated with increased expression of Versican and MT1-MMP and enhanced adhesion

Figure 7

Erk(1/2) activation is highest in the parental Karpas 299 cell line. Cells (8 x 106) were grown in serum free media for 48 hrs, centrifuged at low speed to remove cells and debris, then at 100,000 x g for 1 hr. Vesicles were suspended in PBS and assayed for protein. Equal amounts of protein (5 μg) were loaded in each well of a 7.5% SDS gel. Following transfer to nitrocellulose, blots were probed with anti-MT1-MMP antibody (top) or anti-phospho-p44/42 MAPK antibody (middle), stripped, and reprobed with anti-p44/42 MAPK antibody (next to bottom). The blot was also probed with anti-α5 integrin antibody (bottom). Data are representative of two independent experiments.

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