Figure 5

Karpas 299 cells and vesicles exhibit greater collagenase I activity than either Dep1 or 6RD3 cells. A. Collagen I degradation was monitored in live cells migrating through a native 3D collagen substrate. FITC-collagen type I from bovine skin was copolymerized with rat-tail collagen I. After 48 hr, cells and solid phase collagen were pelleted and the supernatant analyzed for FITC release. B. Collagen I degradation was also measured in vesicles isolated from conditioned media of cells grown for 48 hrs on collagen I. Two independent assays are shown for the intact cells (A) and three independent assays for the vesicles (B). Error bars are standard error of the mean.