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Figure 3 | BMC Cancer

Figure 3

From: CD26 Expression on T-Anaplastic Large Cell Lymphoma (ALCL) Line Karpas 299 is associated with increased expression of Versican and MT1-MMP and enhanced adhesion

Figure 3

Surface expression of MT1-MMP is higher in Karpas parental cells than in Dep1 (CD26 depleted) or 6RD3 (versican depleted). A. Cells were grown overnight on collagen I plates, then biotinylated using an impermeable reagent. Lysates (1 mg protein) were applied to streptavidin-agarose spin columns, washed, and eluted with sample buffer. Eluates were run on 7.5% SDS gels, transferred to nitrocellulose, and probed with MT1-MMP antibodies. B. Flow cytometry of cells grown with and without collagen I. Data are representative of two independent experiments for panel A and for panel B.

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