Figure 2

Confirmation of Versican expression in Karpas 299 cells and in CD26-depleted and Versican-depleted Karpas cells. A. Western blots confirmed versican expression in Karpas cell lines and clones resulting from knockdown of versican in parental Karpas 299 cells using shRNA. Whole cell lysates (30 μg) of Karpas, Dep1, Dep2, and two clones derived from knock down of versican in parental Karpas cells, 1A12 and 6RD3 were run on 7.5% gels. The top of the gel and 250 kD marker are indicated. Blots were probed with anti-versican antibody at 1:100 dilution, followed by anti-mouse HRP at 1:10,000 dilution. B. RT-PCR using V0 and V1 specific primers show product was present when RNA from the parental Karpas 299 cells was used but barely detectable when RNA from Dep1 or Dep2 was used as the template. Results from Western blots and RT-PCR were obtained from two independent experiments.