Skip to main content

Table 2 COX-1, COX-2, and EGFR mRNAs and corresponding protein content in human colon mucosa compared to tumor tissue

From: EGF receptor and COX-1/COX-2 enzyme proteins as related to corresponding mRNAs in human per-operative biopsies of colorectal cancer

 

Tumor tissue

ANOVA

 

Mucosa

Stadium I

Stadium II

Stadium III

Stadium IV

Tumor vs mucosa

p value

Stage I-IV

p value

RNA (n)

(26)

(5)

(8)

(10)

(4)

  

COX-1

10.45 ± 1.59

2.82 ± 0.90

3.40 ± 0.71

1.57 ± 0.34

7.04 ± 2.42

<0.0001

ns

COX-2

0.50 ± 0.21

0.27 ± 0.13

0.81 ± 0.21

1.02 ± 0.39

1.58 ± 0.68

ns

ns

EGFR

13.66 ± 1.26

4.90 ± 1.40

5.28 ± 0.89

3.37 ± 0.58

7.02 ± 0.83

<0.0001

ns

Protein (n)

(30)

(5)

(10)

(10)

(5)

  

COX-1

2.43 ± 0.19

1.97 ± 0.50

1.92 ± 0.38

2.98 ± 0.61

1.97 ± 0.50

ns

ns

COX-2 total*

1.24 ± 0.10

1.53 ± 0.36

3.57 ± 0.68

2.34 ± 0.26

3.42 ± 0.70

<0.0001

I,II 0.002

       

I,IV 0.009

COX-2 66 kDa

1.00 ± 0.13

1.25 ± 0.33

1.63 ± 0.51

1.59 ± 0.32

1.62 ± 0.22

0.028

ns

COX-2 74 kDa

0.20 ± 0.02

0.25 ± 0.09

1.70 ± 0.68

0.70 ± 0.22

1.49 ± 0.82

0.003

I,II 0.014

       

II,III 0.036

EGFR extra

0.64 ± 0.06

0.91 ± 0.09

0.43 ± 0.12

0.40 ± 0.10

0.44 ± 0.15

ns

I,II 0.009

       

I,III 0.006

       

I,IV 0.023

EGFR intra

0.54 ± 0.09

0.40 ± 0.19

0.14 ± 0.06

0.54 ± 0.16

0.42 ± 0.15

ns

ns

  1. Mean ± SEM, RNA: units/units ((GAPDH + 18SRNA)/2), protein arbitrary units. *COX-2 total=66 kDa + ~74 kDa protein band in WB. EGFR intra./EGFR extra. = ab directed towards intracellular/extracellular EGFR protein.