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Table 2 COX-1, COX-2, and EGFR mRNAs and corresponding protein content in human colon mucosa compared to tumor tissue

From: EGF receptor and COX-1/COX-2 enzyme proteins as related to corresponding mRNAs in human per-operative biopsies of colorectal cancer

  Tumor tissue ANOVA
  Mucosa Stadium I Stadium II Stadium III Stadium IV Tumor vs mucosa
p value
Stage I-IV
p value
RNA (n) (26) (5) (8) (10) (4)   
COX-1 10.45 ± 1.59 2.82 ± 0.90 3.40 ± 0.71 1.57 ± 0.34 7.04 ± 2.42 <0.0001 ns
COX-2 0.50 ± 0.21 0.27 ± 0.13 0.81 ± 0.21 1.02 ± 0.39 1.58 ± 0.68 ns ns
EGFR 13.66 ± 1.26 4.90 ± 1.40 5.28 ± 0.89 3.37 ± 0.58 7.02 ± 0.83 <0.0001 ns
Protein (n) (30) (5) (10) (10) (5)   
COX-1 2.43 ± 0.19 1.97 ± 0.50 1.92 ± 0.38 2.98 ± 0.61 1.97 ± 0.50 ns ns
COX-2 total* 1.24 ± 0.10 1.53 ± 0.36 3.57 ± 0.68 2.34 ± 0.26 3.42 ± 0.70 <0.0001 I,II 0.002
        I,IV 0.009
COX-2 66 kDa 1.00 ± 0.13 1.25 ± 0.33 1.63 ± 0.51 1.59 ± 0.32 1.62 ± 0.22 0.028 ns
COX-2 74 kDa 0.20 ± 0.02 0.25 ± 0.09 1.70 ± 0.68 0.70 ± 0.22 1.49 ± 0.82 0.003 I,II 0.014
        II,III 0.036
EGFR extra 0.64 ± 0.06 0.91 ± 0.09 0.43 ± 0.12 0.40 ± 0.10 0.44 ± 0.15 ns I,II 0.009
        I,III 0.006
        I,IV 0.023
EGFR intra 0.54 ± 0.09 0.40 ± 0.19 0.14 ± 0.06 0.54 ± 0.16 0.42 ± 0.15 ns ns
  1. Mean ± SEM, RNA: units/units ((GAPDH + 18SRNA)/2), protein arbitrary units. *COX-2 total=66 kDa + ~74 kDa protein band in WB. EGFR intra./EGFR extra. = ab directed towards intracellular/extracellular EGFR protein.