Figure 5From: Overexpression of heat shock transcription factor 1 enhances the resistance of melanoma cells to doxorubicin and paclitaxel Expression of several ABC transporters is increased in cells overexpressing full HSF1 or its dominant negative form. Changes in ABC transporter genes expression were estimated based on semi-quantitative RT-PCR (after gel densitometry) in B16F10 cells (A) or using quantitative RT-PCR in WM793B (B) and 1205Lu (C) human cells. Fold changes were calculated in relation to expression levels in control (Neo) cells (1.0 value represented by a horizontal red line) after normalization against GAPDH gene expression. Results represent mean values ± SD from three experiments.Back to article page