Figure 4

HSF1 silencing does not influence doxorubicin resistance in mouse melanoma B16F10 cells. A. Detection of transcripts of Hsf1 and Hsp genes in cells expressing control scrambled shRNA or HSF1-specific shRNAs (HSF1-1, HSF1-2). Where indicated, cells were subjected to heat shock (HS) for 1 h at 42°C with subsequent recovery at 37°C for 30 minutes. B. Western blot detection of HSF1 and HSPs in cells expressing control scrambled shRNA or HSF1-specific shRNAs (HSF1-1, HSF1-2). HSF1 was detected directly after HS while HSPs were detected after 6 hours recovery. C. Viability of cells treated with various concentrations of doxorubicin for 48 h. Results of the MTT assay are shown in relation to the untreated cells; mean values ± SD from three independent experiments are presented.