Figure 1

Overexpression of HSF1 enhances doxorubicin resistance of melanoma cells. A. Detection of HSF1 and HSP gene transcripts in mouse (B16F10), and human (WM793B and 1205Lu) cells either with stably introduced empty vector (Neo) /control/ or with a vector encoding the full form of human HSF1 (hHSF1). Where indicated, cells were subjected to heat shock (HS) for 1 h at 42°C with subsequent recovery at 37°C for 30 minutes. B. Western blot detection of HSF1 and HSPs in cells modified and treated as above. HSF1 was detected directly after HS while HSPs were detected after a 6-hour recovery. C. Viability of cells treated with various concentrations of doxorubicin for 48 h (B16F10) or 72 h (WM793B and 1205Lu). Results of the MTT assay are shown in relation to the untreated cells; mean values ± SD from three independent experiments are presented (asterisks indicate p < 0.05).