Figure 7

Opposite activation of MTO1 and MRPL41 promoter by E2 in ER+ and ER- breast cancer cells. Upstream regions of MTO1 (from -994 to +18) (A) and MRPL41 (from -1,030 to +1) (B) were placed upstream of the luciferase gene, and luciferase activities were determined from transiently transfected ER+ (MCF7) and ER- (MDAMB231) cells. Cells were not treated (gray bar) or treated (black bar) with estradiol (E2). Each experiment was performed at least three times and the data are presented as the average and standard error after normalization with activity from vectors containing R0 region in each gene.