Figure 5

Chromatin immunoprecipitation (ChIP) analysis of the MTO1 promoter against estrogen receptor (ER)-α. ChIP assays were performed on the MTO1 promoter using anti-ER-α antibody followed by polymerase chain reaction (PCR) to amplify the half-ER-responsive element (1/2ERE) containing sub-regions. (A) Schematic diagram of the MTO1 promoter showing the four ER-responsive element (ERE) groups (R1-R4). The number of triangles denotes a tandem repeat of the 1/2ERE. Plausible binding sites for other transcriptional factors are also indicated. (B and C) Results of ChIP-PCR for the ER+ MCF7 cells (B) and the ER- MDAMB231 cell (C). Cells were not treated (-) or treated (+) with estradiol.