Figure 3

5-Aza-dC induced upregulation of MTO1 and MRPL41. Cultured breast cancer cells were treated with 5-Aza-dC, and methylation and expression levels were examined for MTO1 (A and C) and MRPL41 (B and D) by real-time methylation-specific polymerase chain reaction (PCR) and RT-PCR, respectively. Estrogen receptor (ER) status of each cell line is indicated as (+) or (-). Gray and black bars represent before and after treatment with 5-Aza-dC, respectively. Each sample was examined in three independent reactions, and the average relative level is presented with the standard error.