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Figure 1 | BMC Cancer

Figure 1

From: Knockdown of autophagy-related protein 5, ATG5, decreases oxidative stress and has an opposing effect on camptothecin-induced cytotoxicity in osteosarcoma cells

Figure 1

Camptothecin decreases cell viability and metabolic activity. A, Camptothecin-induced cell death. DLM8 and K7M3 cells were cultured in 12-well plates and treated with CPT as indicated for 48 h. Cell viability was determined by trypan blue exclusion assay. *, p < 0.05, compared with same treatment group. B, Impact of autophagy inhibition on metabolic activity. Cells were grown in a 96-well plate and allowed to grow in normal media to approximately 70% confluency. MTT assay was used to determine metabolic activity. Control values were set to one hundred percent. *, p < 0.05. C, Impact of autophagy inhibition on cell growth. Cells were grown in 12-well plates in normal media followed by cell count at 48 h. *, p < 0.05. Data represents the results of at least three independent experiments, ± SE. p < 0.05 was considered significant.

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