Figure 6
Induction of EMT in Wnt5a-overexpressing pancreatic cancer cells. (a) Immunohistochemical analysis of vimentin and E-cadherin, two EMT markers, in orthotopic pancreatic tumors. Representative immunostaining images showing increased expression of vimentin and decreased expression of E-cadherin in Wnt5a-overexpressing PANC-1 tumors, compared to control tumors derived from empty vector-transfected PANC-1 cells. Scale bar = 50 μm. (b) Cell morphology of PANC-1 cells transfected with Wnt5a-overexpressing plasmid or empty vector under a phase-contrast microscope. Magnification, ×200. (c) Western blot analysis of the expression of indicated EMT markers in mock- and Wnt5a-transfected PANC-1 and BXPC-3 cells. Representative blots of three independent experiments with similar results are shown. GAPDH was used as an internal control. N: nuclear fraction; C: cytoplasmic fraction.