Figure 5

Effects of Wnt5a on invasiveness and metastasis in an orthotopic pancreatic cancer mouse model. Wnt5a-overexpressing or control pancreatic cancer cells were orthotopically injected into the pancreatic parenchyma of nude mice. Macroscopic and microscopic examination of metastases was done as described in Methods. Representative images of H&E staining showing vascular (a), lymphatic (b), and perineural (c) invasion of Wnt5a-overexpressing PANC-1 cells. Scale bar = 50 μm. Macroscopic appearance of the liver (d) and mesentery (e) showing prominent metastasis in the mice injected with Wnt5a-overexpressing PANC-1 cells. (f) Representative micrograph showing H&E-stained sections of control tumors from empty vector-transfected PANC-1 cells. Scale bar = 50 μm.