The effects of B82 on H460 and BEAS-2B cells. A. Inhibitory effects of B82 on the proliferation of H460 cells. H460 cells were seeded on laminin-coated plates of an ACEA RT-CES system at a density of 30,000/well and were continuously monitored up to 96 h. At the time point of 40 h, B82 at 2.5 or 10 μM and vehicle control DMSO were added into the corresponding wells (indicated by color and arrow). The data represent the mean values ± S.D. for triplicate wells. B. B82 induced cell apoptosis in H460 cells. H460 cells were treated with B82 or curcumin at indicated concentrations for 12 h, and then stained with Annexin V and PI, followed by detection using flow cytometry. The representative pictures are shown. C. The percentage of cells with early apoptosis and late apoptosis are shown (n = 4). Data are presented as the mean ± SEM. *p < 0.05, vs. vehicle control; # p < 0.05, vs. curcumin group. D. B82 affects the proliferation of BEAS-2B cells. Cells were treated with B82 at indicated concentrations for 24 h or 72 h, and the cell survival was determined using MTT assay, and the inhibitory rates and IC50 values were calculated.