Figure 5
From: Salinomycin increases chemosensitivity to the effects of doxorubicin in soft tissue sarcomas
Salinomycin sensitizes soft tissue sarcoma cells to doxorubicin induced apoptosis. Each cell line was cultivated in the presence of salinomycin (1 μM); doxorubicin (125 ng/mL), or with a combination of both compounds before cells were subjected to analysis. Early apoptosis events were analyzed by Annexin V+ TO-PRO-3- cell, 24 h post treatment (A). DNA fragmentation was measured by flow cytometry as a sub-G1 cell fraction after propidium iodide staining, 48 h post treatment (B). Error bars denote the standard error (n = 3). (* p < 0.05, ** p < 0.01, *** p < 0.001).