Figure 4

Indirect immunofluorescence assay on microinjected MCF7 cells with VP3 protein and truncated VP3 protein. The MCF7 cells were injected with A) PrVP3, B) PrVP3A1-69N1N2, C) PrVP3A1-46N1N2, D) PrVP3A1-31N1N2 E) PrVP3A32-69N1N2, F) PrVP3A32-62N1N2 and negative control, G) PrMBP. The microinjected cells were assayed at 2 hr and 10 hr. The green fluorescence showed the present of injected protein. At 2 hr, the injected protein was detected in the cytoplasm of the cells (showed in red arrow), whereas the protein was found in the nucleus of the cells at 10 hr (showed in yellow arrow) except PrMBP (G).