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Figure 3 | BMC Cancer

Figure 3

From: Clinical significance of L-type amino acid transporter 1 expression as a prognostic marker and potential of new targeting therapy in biliary tract cancer

Figure 3

Effect of LAT inhibition on in vitro cellular proliferation and anti-tumor activity of GEM and 5-FU: (A) BCH inhibits [14C]L-leucine uptake concentration-dependently in HuCCT1 cells (n = 4). Ordinate shows a percentage of [14C]L-leucine uptake in the absence of BCH as a control. (B) Expression of LAT1, LAT2, LAT3, and LAT4 mRNA in HuCCT1 cells (n = 4). Ordinate shows relative quantity of mRNA calibrated by LAT1 mRNA. (C) BCH decreases number of HuCCT1 cells concentration-dependently (n = 4). Ordinate shows number of cells in a percentage of control (without BCH). Addition of 10 mM BCH enhances anti-tumor effect of GEM (D) and 5-FU (E) on HuCCT1 cells. Ordinate shows number of cells in a percentage of control (n = 4). A statistically significant difference from the control is indicated by *** (P < 0.001).

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