AD 198 exhibited potent anti-tumor activity and suppressed c-Myc expression in TRAF3-sufficient mouse and human B lymphoma cell lines. (A) Anti-tumor activity of AD 198. Mouse B lymphoma cell lines A20.2 J, m12.4.1 and CH12.LX, or human B lymphoma cell lines Daudi, Ramos and JeKo-1 were treated with various concentrations of AD 198 for 24 h. Total viable cell numbers were subsequently determined by MTT assay. The graphs depict the results of three independent experiments with duplicate samples in each experiment (mean ± SEM). (B) Time-dependent effects of AD 198 on c-Myc protein levels. Mouse or human B lymphoma cells were cultured in the absence or presence of AD 198 of indicated concentrations. Total cellular lysates were prepared at indicated time points. Proteins were immunoblotted for c-Myc, followed by caspase 3 and actin. Immunoblots of actin were used as loading control. Results are representative of three independent experiments.