HMEC and 4T1 migration in wound healing assay using ES or ES-based fusion proteins as inhibitors. Pictures were taken at magnification 40X in light microscopy (A and C). Quantification results of migrated cells, counted using the software Image-Pro Plus 6.0, are shown in B and D, assuming control as 100%. Inhibitors were used at indicated concentration. Results shown are average values of 6 representative fields in each of the two different experiments performed in duplicates, and error bars represent SEM. *, P ≤ 0.05, **, P ≤ 0.001, compared with ES, respectively. Cells were viewed with a microscope and pictures were taken at × 40.