Figure 3
From: Chemotherapy-induced hyaluronan production: a novel chemoresistance mechanism in ovarian cancer
Carboplatin treatment increases HA production by ovarian cancer cells. A. HA levels in the CM of ovarian cancer cell lines, determined by ELISA assay. Data represent mean ± SEM from 3 independent experiments performed in duplicate. *, significantly different from no CBP control (P < 0.05, independent t test). B. qRT-PCR for HAS2 expression. C. qRT-PCR for HAS3 expression. Relative gene expression in B and C was determined by calibration against no CBP control and normalized to the house keeping gene β-actin using the 2-∆∆CT method. Data represents mean ± SEM from 2 independent experiments performed in triplicate. *, significantly different from no CBP control (P < 0.05, independent t test). C = no CBP, CBP = carboplatin (LD50 dose), CM = conditioned media.