Figure 1From: Clinical instability of breast cancer markers is reflected in long-term in vitro estrogen deprivation studies Experimental flowchart. ER, PR and HER-2/neu expression were analysed by qRT-PCR and immunocytochemistry (ICC) at selected time points (control, 2 days, 4 days, 1–8 weeks and 10 months after estrogen deprivation). Microarray analysis was performed on control cells and at 2 days, 6 weeks and 10 months after estrogen deprivation.Back to article page