MK801 inhibited growth of hepatocellular carcinoma cells. A: Cell lysates prepared from hepatocellular carcinoma (HepG2, HuH-7, and HLF), colon colorectal carcinoma (HCT-116), and neuroblastoma (SH-SY5Y) were separated on 12.5% SDS-PAGE and western blot analysis was performed with anti-NMDAR1 or anti-β-actin antibody. B: Total RNA was purified from cultured cells and cDNA was synthesized with random hexamers. PCR was performed with NMDAR subtype or β-actin specific primers Amplified products were separated on 1.5% agarose gels. C: Cells were seeded into 96-well plates and different concentrations of MK-801 or NBQX were added to culture medium for 24 h. Cell viability was measured by MTT assay. Each bar represents the mean ± SD of three replicates. D: Various concentrations of MK-801 were added to the medium and cells were cultured for 72 h. Cell viability was measured by MTT assay. Each point represents the mean ± SD of three replicates.