Figure 4

Effects of the combination of ACR and LY294002 on the induction of apoptosis in HLF cells. The cells were treated with vehicle, 1 μM ACR alone, 5 μM LY294002 alone, or a combination of 1 μM ACR and 5 μM LY294002 for 48 or 72 hours. (A) TUNEL assays were performed using cells treated with test drugs for 48 hours. TUNEL-positive cells were counted and examined as the percentage of the DAPI-positive cell number (500 cells were counted in each flask). (B) Caspase-3 activity assays were performed with a fluorometric system using samples treated for 72 hours. # P < 0.01. * P < 0.05.