Figure 2

Inhibition of cell growth by ACR alone, LY294002 alone, or various combinations of these agents in human HCC-derived cells and Hc normal hepatocytes. (A) HLF human HCC cells were treated with the indicated concentrations of ACR alone, LY294002 alone, and various combinations of these agents for 48 hours. (B) The data obtained in (A) was used to calculate the combination index. (C) Huh7, Hep3B, and HepG2 human HCC cells were treated with vehicle, 1 μM ACR alone, 5 μM LY294002 alone, or a combination of 1 μM ACR and 5 μM LY294002 for 48 hours. (D) Hc human hepatocytes were treated with the indicated concentrations of ACR alone, LY294002 alone, and various combinations of these agents for 48 hours. (A), (C), and (D) Cell viability was determined by the MTS assay and expressed as a percentage of the control value. Error bars present the SD of triplicate assays. * P < 0.05.