Figure 4

Effect of HOTAIR on cell migration, invasion and metastasis. (A, B) SPC-A1 cells were transfected with HOTAIR siRNA or si-NC, and A549 cells were transfected with pCDNA-HOTAIR or pCDNA-NC. Transwell assays were performed to investigate the migratory and invasive ability of NSCLC cells. (C) Analysis of an experimental metastasis animal model was performed by injecting HOTAIR knockdown SPC-A1 cells into nude mice through the lateral tail vein. Images of lungs from mice in each experimental group are shown. The numbers of tumor nodules on lung surfaces are shown. ** P < 0.01.