Figure 4

MT1G inhibited the migration and invasion of thyroid cancer cells. K1 cells stably transfected with pEGFP-N1-MT1G or empty vector were starved overnight and then seeded in the Transwell chambers without Matrigel for migration assay (A), and coated with Matrigel for invasion assay (B), respectively. Following a 24 h-culture, non-migrating (or non-invading) cells in the upper chamber were removed and migrating (or invading) cells were stained and calculated in four microscopic fields per sample. Shown are representative images of migrating (or invading) cells (left panels). The bar graphs (right panels), corresponding to left panels, show means ± SD of the numbers of migrating (or invading) cells from three independent experiments. **, P <0.01; ***, P <0.001. (C) FTC133 cells were transfected with pEGFP-N1-MT1G or empty vector. After 48 h of post-transfection, the scratch wound-healing assay was performed to evaluate the effect of MT1G on cell migration. Representative images of cell migration of FTC133 cells in scratch wound-healing assay were shown in left panel. The bar graphs show means ± SD. of gap width of wounds at the indicated times from three independent experiments (right panel). *, P <0.05.