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Table 3 Methylation status of 12 genes in normal tissue, DCIS and invasive breast cancer patients

From: Quantitative DNA methylation analyses reveal stage dependent DNA methylation and association to clinico-pathological factors in breast tumors

  Normal   DCIS Invasive
   I   II   III   IV  
GENE Methylation status N % Sample number % Sample number % Sample number % Sample number % Sample number %
ABCB1 Hypomethylated 0 0 0   0   0   0   0  
Normal like 6 100 15 57.7 33 52.4 6 30 31 36 6 30
Hypermethylated 0   11 42.3 30 47.6 14 70 57 64 14 70
BRCA1 Hypomethylated 0 0 0 0 2 3 2 10 10 12.8 1 5.9
Normal like 6 100 26 100 63 97 18 90 68 87.2 16 94.1
Hypermethylated 0   0 0 0   0   0   0  
CDKN2A Hypomethylated 0 0 0 0 0   0   0   0  
Normal like 6 100 26 100 60 92.3 18 90 61 67.8 14 70
Hypermethylated 0   0   5 7.7 2 10 29 32.2 6 30
ESR1 Hypomethylated 0 0 0 0 0   0   0   0 0
Normal like 6 100 26 100 63 97 19 95 63 98.4 19 100
Hypermethylated 0   0   2 3 1 5 1 1.6 0  
FOXC1 Hypomethylated 0 0 0   0   0   0   0  
Normal like 6 100 6 23 34 52.3 7 35 40 46.5 10 47.6
Hypermethylated 0   20 77 31 47.7 13 65 46 53.5 11 52.4
GSTP1 Hypomethylated 0 0 1 3.8 0   0   0   0  
Normal like 6 100 10 38.5 27 41.5 5 25 29 33 5 25
Hypermethylated 0   15 57.7 38 58.5 15 75 59 67 15 75
IGF2 Hypomethylated 0 0 11 42.3 25 38.4 6 30 34 39.1 9 47.4
Normal like 6 100 14 53.8 32 49.2 9 45 32 36.8 5 26.3
Hypermethylated 0 0 1 3.9 8 12.4 5 25 21 24.1 5 26.3
MGMT Hypomethylated 0 0 0   13 20.3 13 65 16 24.2 8 44.4
Normal like 6 100 25 96.1 50 78.1 5 25 35 53.1 5 27.7
Hypermethylated 0   1 3.9 1 11.6 2 10 15 22.7 5 27.7
MLH1 Hypomethylated 0 0 4 15.4 12 18.5 6 32 4 4.5 3 17.6
Normal like 6 100 21 80.8 47 72.3 10 53 68 77.3 13 76.5
Hypermethylated 0   1 3.8 6 9.2 3 16 16 18.2 1 5.9
PPP2R2B Hypomethylated 0 0 0   0   0   0   0  
Normal like 6 100 7 26.9 15 24.6 6 35 40 30.8 3 16.7
Hypermethylated 0   19 73.1 46 75.4 11 65 45 69.2 15 83.3
PTEN Hypomethylated 0 0 0   0   0   0   0  
Normal like 6 100 21 80.8 56 86.2 13 65 23 27.7 9 45
Hypermethylated 0   5 19.2 9 13.8 7 35 60 72.3 11 55
RASSF1A Hypomethylated 0 0 2 7.7 2 3.1 1   0   0  
Normal like 6 100 2 7.7 10 15.4 2 11 3 4.8 1 5.6
Hypermethylated 0   22 84.6 53 81.5 17 90 60 95.2 17 94.4
  1. Samples were considered as hypermethylated if the% DNA methylation was higher than the sum of two times the standard deviation and mean of the normal samples and hypomethylated if% DNA methylation was lower than two times the standard deviation - mean of the normal samples.