Immunblots with the antibody against Sirt1 for MiaPaCa-2 and PANC-1. A) Endogenous protein levels showed a comparably weaker immunosignal than cells overexpressing Sirt1. B) The blots were scanned and analysed quantitatively using ImageJ. The values were normalized for pGFP. The graphs show strongly increased immunosignal densities for cells that overexpress Sirt1.