Effects of cofilin knockdown on EGF-induced migration in human bladder cancer T24 cells. (A) The effect of EGF on cell migration. Cells were treated with EGF (1–100 ng/mL) for 80 min and migration was quantified with a Boyden microchemotaxis chamber assay. (B) Non-phosphorylated and phosphorylated cofilin expression in human bladder cancer T24 cells transfected with small interfering (si)RNA for cofilin. (C) Effects of siRNA-cofilin transfection on EGF-induced migration. The transfected cells were treated with EGF (50 ng/mL) and then subjected to migration assays. Migration in the quiescent state is expressed as 100%. * p < 0.05. Con, control; NS, nonsilencing siRNA; siRNA, siRNA for cofilin; p-cofilin, phosphorylated cofilin.