Expression and phosphorylation levels of cofilin in bladder tissues isolated from NMIBC, and MIBC tissues. (A) Enlargement of cofilin spots (arrows) from 2-DE gel images obtained from normal bladder tissue, NMIBC and MIBC samples. (B) Western blot analysis of proteins extracted from normal human bladder, NMIBC and MIBC samples. (CE) The statistical results obtained from panel B. Expression (C), phosphorylation (D) and ratio (E) of phosphorylated to total cofilin. * p < 0.05. White bars (a), normal bladder tissue (control); striped bar (b), NMIBC tissue; black bars (c), MIBC tissue.