Figure 4

MiR-302b inhibits cell proliferation through EGFR-dependent cell cycle regulation. A- Western blot results of miR-302b-targeted EGFR and non-direct targeted cell cycle regulation proteins in SMMC-7721 cells after transfection of miR-302b over-expression construct and miR-ctrl. β-actin was used as an internal control. B- Protein level of EGFR and relative cell cycle regulation proteins were measured by western blot 48 h after transfection of siEGFR and siRNA-ctrl into SMMC-7721 cells. The expression of the proliferation-related gene was normalized to β-actin. The intensity for each band was quantified. The value under each lane indicates the relative expression level of the proliferation-related gene, which is represented by the intensity ratio between miR-302b and miR-ctrl or inhibitor and inhibitor-ctrl groups.