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Figure 1 | BMC Cancer

Figure 1

From: MicroRNA-302b suppresses cell proliferation by targeting EGFR in human hepatocellular carcinoma SMMC-7721 cells

Figure 1

Dysregulated miR-302b/EGFR in hepatocarcinoma tissues and cells. A- qRT–PCR analysis of miR-302b expression in 27 paired HCC tissues and their corresponding adjacent nontumorous livers. The expression of miR-302b was normalized to U6 snRNA. The data are reported as mean ± s.d. (*P < 0.05, Student’s t-test). B- qRT–PCR analysis of EGFR expression in 27 paired primary HCC tissues and their corresponding adjacent nontumorous liver tissues. The expression of EGFR was normalized to β-actin (*P < 0.05, Student’s t-test). C- qRT–PCR analysis of miR-302b expression in normal hepatocytes (HL-7702 cells) and HCC cells (Bel7402, Hep3B, HepG2, SMMC-7721 cells), (**P < 0.01, *P < 0.05, Student’s t-test). D- Western blot analysis of EGFR expression in four pairs of HCC tissues and their corresponding nontumorous liver tissues, with β-actin as an internal control. The intensity for each band was quantified. The value under each lane indicates the EGFR expression in four tumor tissues compared with their corresponding nontumorous liver tissues. E- Western blot analysis of EGFR expression in hepatocytes, and hepatoma cells, with β-actin as an internal control. The value under each lane is represented the intensity ratio between hepatoma cells and hepatocytes.

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