Figure 8

Ratio of HER2, HPSE1 and Syn-1 mRNA expression of trastuzumab treated per non-treated breast cancer cells MCF7, MCF7-HPSE1 and SKBR3. These cells were treated for three days with 25 μg/mL of trastuzumab. The RNA was extracted using TRIzol reagent, converted into cDNA by RT-PCR and submitted to qRT-PCR using specific primers for HER2, Syn-1 and HPSE1. The mRNA expression of constitutive genes GAPDH and RPL13A was used to correct the mRNA expression of HER2, Syn-1 and HPSE1, as described in methods. Each bar indicates the mean ± SD of triplicate assays. *P < 0.05 versus non-treated MCF7 cells and ** P < 0.05 versus non-treated MCF7-HPSE1.