Figure 5
From: Heparan sulfate mediates trastuzumab effect in breast cancer cells
HPSE1, HER2 and Syndecan-1 mRNA expression in MCF10A, SKBR3, MCF7 and MCF7-HPSE1. The RNA was extracted using TRIzol reagent, converted into cDNA by RT-PCR and submitted to qRT-PCR using specific primers for HER2, Syn-1 and HPSE1, as described in methods. The values were corrected by RPL13a (ribosomal protein) and GAPDH expressions. Each bar indicates the mean ± SD of triplicate assays. *P < 0.05 compared to MCF10A and **P < 0.001 compared to MCF7.