Semi-quantitative analysis of the ERGIC3 protein by western blot. (A) The averages of protein levels in the 15 tumor tissues (T) and their adjacent nonmalignant tissues (N). (B) The averages of protein levels in three separate experiments on SPCA-1, GLC-82, EPLC-32M1 and BEAS-2B. The Y-axis shows the ratio of the ERGIC3 grey value divided by the beta-actin grey value. The significance was evaluated by a paired t-test (two tailed). *: P < 0.05.