Figure 5From: RETRACTED ARTICLE: Critical role of CCDC6 in the neoplastic growth of testicular germ cell tumors Analysis of CCDC6 expression in normal human testis and testicular germ cells tumors. (A) 40 ug of total tissue lysates were resolved on 10% SDS-PAGE transferred onto nitrocellulose filters and western blotted with α-CCDC6 antibody. NT, normal testis; lanes 2–6: seminomas; lane 7, teratoma; lane 8, embryonal carcinoma; lane 9, TCAM2 seminoma cell line. Anti-actin hybridization is shown as loading control. The blots are representative of three independent experiments. (B) Top: CCDC6 relative expression in TCAM2 and GC1 cells analysed by qRT-PCR. Data are the mean +/− SD of three independent experiments. Bottom: CCDC6 expression in the unique TCAM2 seminoma cells and GC1 Cells. Whole lysates were detected by western blotting with anti-CCDC6 antibody. (C) the activation of the molecules of the DNA Damage Response were monitored using the anti-pS1981_ATM (pATM), the anti-pT68_Chk2 (pChk2), and the anti-pS139_H2AX (γH2AX). Total ATM, Chk2 and H2AX are shown. Before lysis, the cells have been treated 1 hour with H2O2 (10 μM), and after washing out the hydrogen peroxide the cells have been left an additional hour before the lysis. Anti-tubulin hybridization is shown as loading control.Back to article page