Figure 1
From: RETRACTED ARTICLE: Critical role of CCDC6 in the neoplastic growth of testicular germ cell tumors
CCDC6 expression in adult mouse testis. (A) Immunohistochemical staining for CCDC6 protein in adult mouse testis (LSAB tecnique). A representative seminiferous tubule showing staining in the basal germ cells (arrow heads), Sertoli cells and Leydig cells. (B) Distribution of CCDC6 protein in mouse testicular cells. Western blot analysis of CCDC6 protein in mouse adult testis (lane 1), interstitium (lane 2), Sertoli cells (lane 3) and normal mouse germ cells (lanes 4–7) (40 μg/lane). Whole lysates were detected by western blotting with anti-CCDC6 polyclonal serum or with anti-ERK1 antibodies used as an internal standard. ERK1 antibodies recognize both ERK1 and ERK2, which are expressed at similar levels in all cell types with the exception of spermatozoa. Anti-tubulin hybridization is shown as loading control.