Effects of stable silence of Tβ10 on tumor metastasis of KKU-M214 cell line in nude mice. (A) M214-sh-vector-GFP cells or M214-sh-Tβ10-GFP cells were injected orthotopically into the spleen of nude mice for 3 weeks. Spleen (top), right and caudate lobes of liver (middle), medial and left lobes (bottom) were excised and GFP expressing tumors (black arrows) were examined using an UV illuminating system. (B) Graph showing the total number of GFP+ gross liver nodules in individual livers (±SD; n = 4). (C) Representative pictures of liver micrometastasis. Frozen sections of the liver were cut from the caudate and left lobe of each mouse and visualized under a inverted fluorescent microscope using a 10× objective to verified the green signal of GFP; and 6 fields of GFP indicated area of each liver section were taken (top) and stained for H&E (bottom). (D) The number of liver micrometastasis foci was quantified (±SD; n = 4, *P<0.05 versus the control). (E) Tβ10 silence persisted in the M214 sh-Tβ10-GFP cell line-derived tumors compared with M214 sh-vector-GFP cell line-derived tumors by real-time RT-PCR.