Figure 4
From: Partial loss of Smad signaling during in vitroprogression of HPV16-immortalized human keratinocytes
Time course of Smad2 phosphorylation after TGF-β treatment of normal HKc, low-passage HKc/HPV16 and HKc/DR. Normal HKc, low-passage (< 40) HKc/HPV16 and HKc/DR were treated with 40 pM TGF-β for the times indicated. Whole-cell lysates were analyzed for phospho-Smad2 by Western blot analysis. Total protein (25 μg/well) was loaded and resolved in a 10% polyacrylamide gel. After transfer to a PVDF membrane, phospho-Smad2 was detected with a rabbit anti-human phospho-Smad2 antibody. Representative time courses are shown for normal HKc (panel A), low-passage (<40) HKc/HPV16 (panel B) and HKc/DR (panel C). Actin was used as a loading control.