Figure 2From: Partial loss of Smad signaling during in vitroprogression of HPV16-immortalized human keratinocytes Nuclear accumulation of Smad3 and Smad4 in HKc/HPV16 after treatment with TGF-β. HKc/HPV16 were immunostained for Smad3 (Cy3, red) and for Smad4 (Alexa Fluor 488, green) at various times (0 to 60 min) after treatment with 40 pM TGF-β. Nuclei were visualized with DAPI (blue).Back to article page