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Figure 1 | BMC Cancer

Figure 1

From: Partial loss of Smad signaling during in vitroprogression of HPV16-immortalized human keratinocytes

Figure 1

Western blot analysis of Smads during in vitro progression of HKc/HPV16. Smad protein levels in whole-cell extracts prepared from normal HKc, low passage (less than 40) and high passage (greater than 100) HKc/HPV16, HKc/GFI, and HKc/DR were determined by Western blotting. Total protein (30 μg per well) was loaded and resolved in a 10% polyacrylamide gel. After transferring proteins to PVDF membranes, the Smads were detected using anti-Smad antibodies: A – Smad2, B – Smad3, C – Smad4, and D – Smad7. Actin was used as a loading control.

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