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Figure 2 | BMC Cancer

Figure 2

From: Exosome derived from epigallocatechin gallate treated breast cancer cells suppresses tumor growth by inhibiting tumor-associated macrophage infiltration and M2 polarization

Figure 2

Exosomes-derived from EGCG-treated tumor cells down-regulate chemo-attractants in tumor cells, and suppress M2 polarization of tumor-associated macrophages in ex vivo model. (A) Tumor cells were isolated from established murine breast cancer using 4T1 cell line as in Figure 1, and were treated with EGCG (100 μM). After 24 hours of incubation, total RNA from cells were extracted and subjected to RT-qPCR to detect the levels of CSF-1, CCL-2, and GAPDH. Histogram shows the relative expression of molecules compared to GAPDH as internal control. (B) Tumor-associated macrophages were isolated from established murine breast cancer using 4T1 cell lines, and were treated with exosomes (50 μg/ml) derived from EGCG-treated or untreated 4T1 cells. After 24 hours of incubation, total RNA from cells were extracted and subjected to RT-qPCR to evaluate the levels of M2high cytokines (IL-6 and TGF-β) and M2low cytokine (TNF-α). Histogram shows the relative expression of molecules compared to GAPDH as internal control. (C) Tumor-associated macrophages were isolated from established murine breast cancer cell lines, and treated with exosomes (50 μg/ml) derived from EGCG-treated or untreated 4T1 cells. After 24 hours of incubation, total lysates from cells were extracted and subjected to Western-blot to evaluate the protein levels.

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