Figure 6

Histological characterization of DMBA-induced tumors. The breast tumors were isolated on day 15 after the first appearance of tumor and embedded in OCT. Transverse serial sections of tumor tissues (10 μm) were prepared using a cryostat (Leica CM1900, Leica Microsystems, Bannockburn, IL) and the sections were subjected to H & E stain (Sigma Chemical Co., St Louis, MO). The tumors were classified based on their morphological features as described by Dunn [45].