Figure 2

Effect of DHA, CCM, and DHA + CCM on DMBA-induced breast tumor development. After an acclimation period, SENCAR mice (NIH, Fredric, MD) were divided into 4 groups (15/group). Each group was fed a different diet for 3 weeks prior to tumor induction: 1) 18% corn oil (Brown line); 2) 15% DHASCO (DSM, Columbia, MD) + 3% corn oil (Light green line); 3) 18% corn oil + 0.2% curcumin (Orange line); or 4) 15% DHASCO + 3% corn oil + 0.2% curcumin (Dark green line). Doses of DHA and CCM were selected based on published data [2]. Mice continued to be fed the corresponding diet during the entire course of the experiment. Each mouse was gavaged with 200 μl DMBA (1 mg/ml in sesame oil) once every week for 6 weeks to induce breast tumors. The appearance of palpable tumors was monitored daily beginning with the first DMBA gavage. The statistical analysis and characterization of the effects of different diets on DMBA-induced breast tumors are shown in Table 4.