Figure 5

Effect of IGF-I and EGF 20 nM (for 15 min) on downstream signaling pathways in all pancreatic cancer cell lines used in this study. Cells were grown to near-confluency in 10% FBS growth followed by 24 h incubation in 0.1% FBS growth medium at 37°C. Following that, cells were stimulated with 20 nM of EGF and IGF-I for 15 min. Cells were lysed, protein samples were separated by SDS-PAGE, transferred onto PVDF membranes and probed with the antibodies of interest.