Spatial localization of cohesion-related proteins in L-540 cells. Indirect immunofluorescence was employed to determine the spatial localization of six proteins involved in sister chromatid cohesion (SMC1A, SMC3, STAG2, RAD21, Securin and Separase). Presented are representative high-resolution (63×) images obtained from L-540 cells in interphase. Nuclei were counterstained with DAPI and immunofluorescently labeled for the epitope indicated on the left. Scale bar represents 3 μm.