HL cell lines exhibit cohesion defects. (A) Representative images of mitotic chromosome spreads from the L-540 cells demonstrating normal primary constriction cohesion (Upper left) and the various categories of aberrant cohesion; PCGI (Mild), PCGII (Moderate), and PCGIII (Severe). Each cohesion category (quadrant) contains a low-resolution DAPI image of the entire mitotic spread. Each spread contains a white bounding box that defines the region magnified and presented on the right-hand side of each quadrant. Scale bar represents 10 μm. (B) Graphs depicting the total fraction of cells with aberrant cohesion; white (normal), black (aberrant). The total number of mitotic spreads evaluated for each cell line is indicated at the bottom of each column. (C) Graphical depiction for only the fraction of mitotic spreads with cohesion defects. The frequencies of defects are classified according to three different aberrant cohesion categories (e.g. PCGI - light gray; PCGII - dark gray; and PCGIII - black). The total number of mitotic spreads exhibiting aberrant cohesion is indicated at the base of each column.