Self-renewal and the phenotype of GSCs are disrupted upon Nrf2 knockdown. (A) Photo of cloning efficiency of GSCs transduced with either Scrambled or Nrf2 shRNA lentiviral constructs. (B) gentian violet positive colonies in 20 random 4X field. (C) Representative photomicrographs of sphere-like (left) and mixed-differentiated (right) colonies of cells. (D) Quantification of sphere-like and mixed-differentiated-like colonies. The proportion of spheres like colonies is lower in Nrf2-downregulated group.